spectral dapi (Akoya Biosciences)

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Akoya Biosciences spectral dapi

Example images of immunofluorescent staining on a pancreatic cancer core sample from a tissue microarray from different antibodies showing pan-cytokeratin (red, Opal 570, top left), SMAD4 (green, Opal 620, top right), and an unnamed biomarker (yellow, Opal 520, bottom left). The merged images are shown in the bottom right. Nuclei were stained blue with <t>DAPI.</t> Total cellular fluorescence intensity units of SMAD4 per cell in the pan-cytokeratin positive cells were determined by a Vectra Polaris microscope and Inform software.

Spectral Dapi, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 99/100, based on 1317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spectral+dapi/Opal+480/pmc13641179-52-4-6
Average 99 stars, based on 1317 article reviews

spectral dapi - by Bioz Stars, 2026-11

99/100 stars

Images

1) Product Images from "High SMAD4 Expression is Associated with Improved Clinical Outcomes in Patients with Resectable Pancreatic Cancer: An Analysis of NRG Oncology/RTOG 9704"

Article Title: High SMAD4 Expression is Associated with Improved Clinical Outcomes in Patients with Resectable Pancreatic Cancer: An Analysis of NRG Oncology/RTOG 9704

Journal: International journal of radiation oncology, biology, physics

doi: 10.1016/j.ijrobp.2026.09.014

Figure Legend Snippet: Example images of immunofluorescent staining on a pancreatic cancer core sample from a tissue microarray from different antibodies showing pan-cytokeratin (red, Opal 570, top left), SMAD4 (green, Opal 620, top right), and an unnamed biomarker (yellow, Opal 520, bottom left). The merged images are shown in the bottom right. Nuclei were stained blue with DAPI. Total cellular fluorescence intensity units of SMAD4 per cell in the pan-cytokeratin positive cells were determined by a Vectra Polaris microscope and Inform software.

Techniques Used: Staining, Tissue, Microarray, Biomarker Discovery, Fluorescence, Microscopy, Software

Incubation:

Article Title: Combinatorial delivery of low-dose radiotherapy and immunotherapy to patients with immune-excluded tumors enhances CD8+ T cell functionality
Article Snippet: C C R - 2 5 - 2 7 4 3 / 3 7 7 1 1 1 5 / c c r - 2 5 - 2 7 4 3 . p d f b y g u e s t o n 2 2 A p r i l 2 0 2 6 10 incubation, secondary HRP-labeled antibody incubation for 16 minutes (Discovery OmniMap anti-rabbit HRP (Ventana, RRID: 760-4311) or anti-mouse HRP (Ventana, RRID: 760-4310), OPAL TM reactive fluorophore detection (Akoya Biosciences, Marlborough, MS, USA) that covalently label the primary epitope (incubation : 12 minutes) and then antibodies heat denaturation. .. Nuclei were visualized by a final incubation with Spectral DAPI (1/10, RRID: FP1490, Akoya Biosciences) for 12 minutes. ..

other:

Article Title: Targeting peptidyl-arginine deiminase 4 suppresses SARS-CoV-2 replication and modulates the inflammatory response
Article Snippet: Spectral DAPI , Akoya Biosciences , Cat# FP1490.

Article Title: High-Dimensional Protein Analysis Uncovers Distinct Immunologic and Stromal Features in Primary and Metastatic Pancreatic Ductal Adenocarcinoma
Article Snippet: Antibody dilutions used Diamond Antibody Diluent (Cell Marque, #938B-09).

Immunohistochemistry:

Article Title: CD8 + T cells sustain vaccination-induced immunity against dissemination of contained tuberculosis in immunosuppressed hosts
Article Snippet: .. The 9-colour Opal IHC panel included the following antibodies: Mtb (Cat. No. ab905, clone pAb, Abcam), CD3 (Cat. No. A045229-2, clone pAb, Dako), CD4 (Cat. No. 14-9766-82, clone 4SM95, Invitrogen), CD8a (Cat. No. HS361-008, clone Rb321E9, Synaptic systems), B220 (Cat. No. 103202, clone RA3-6B2, Biolegend)), CD161c/NK1.1 (Cat. No. 24395S, clone E6Y9G, Cell Signalling Technology), CD11c (Cat. No. 97585, clone D1V9Y, Cell Signalling Technology), CD11b (Cat. No. ab133357, clone EPR1344, Abcam), and Spectral DAPI (Cat. No. FP1490, Akoya Bioscience). .. cLNs were stained using the NanoString GeoMx Mouse Whole Transcriptome Assay (version 3.0.0.182) by NanoString Technologies, Inc. (Seattle, Washington, USA).

Staining:

Article Title: Conventional type-1 DC density is associated with checkpoint inhibitor response across multiple types of cancer
Article Snippet: Afterwards, tyramide signal amplification (TSA) was performed using Opal fluorophores (570, 520, 620, and 480, catalogue numbers #FP1488001KT FP1487001KT, FP1495001KT and FP1500001KT, respectively) diluted in 1X Plus Amplification Diluent (catalogue #FP1609, Akoya Biosciences). .. The final round included nuclear staining using spectral DAPI (catalogue #FP1490, Akoya Biosciences). .. Afterwards, the coverslip was placed using Faramount Aqueous Mounting Medium (catalogue #S3025, Dako-Agilent).

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